Synonyms
FLJ14824 antibody, RAB2B antibody, RAS family, member RAB2B antibody, ras-related protein Rab-2B antibody, GTP-binding protein RAB2B antibody, RAB2B, member RAS oncogene family antibody
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
24 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 216 of RAB2B (Uniprot ID# Q8WUD1)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

RAB2B antibody detects RAB2B protein at cytoplasm and membrane on Liver carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded Liver carcinoma.
RAB2B antibody (107-10783) dilution: 1:250.

Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
107-10783 diluted at 1:1000

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RAB2B(107-10783) antibody at 1:200 dilution.

Sample(30 ug whole cell lysate)
A: Hep G2
12% SDS PAGE
107-10783 diluted at 1:500
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.