Synonyms
RAB3C antibody, ras-related protein Rab-3C antibody, "RAB3C, member RAS oncogene family antibody"
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
26 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 227 of RAB3C (Uniprot ID# Q96E17)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:1000-1:10000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
107-10784 diluted at 1:5000

Immunohistochemical analysis of paraffin-embedded DLD1 xenograft, using RAB3C(107-10784) antibody at 1:500 dilution.

Sample (30 ug of whole cell lysate)
A: H1299
12% SDS PAGE
107-10784 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.