Rabbit Anti-Human Rab5 (C-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P20339 |
| Gene Id | 5868 |
| Gene Symbols | RAB5A |
| Protein Name / Synonyms | Rab5 (C-term) |
| Clonality | Polyclonal |
| Preparation | This Rab5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 182-211 amino acids from the C-terminal region of human Rab5. |
| Concentration (lot specific) | 0.440 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, IF, FC |
| Research Area | Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
Rab5 is required for the fusion of plasma membranes and early endosomes.
Specificity
Other Names
RAB5A; RAB5; Ras-related protein Rab-5A
NCBI Accession #
NP_004153.2
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
23659
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100; IF: 1:10-50; FC: 1:10-50
 | Western blot analysis of Rab5 Antibody (C-term) (Cat# 102-17043) in HL-60 cell line lysates (35ug/lane). RAB5 (arrow) was detected using the purified Pab. |
 | Formalin-fixed and paraffin-embedded human brain tissue with Rab5 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
 | Immunofluorescence analysis of Rab5 Antibody (C-term) with paraffin-embedded human brain tissue . 0.025 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI. |
 | Rab5 Antibody (C-term) (Cat# 102-17043) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.