RAB6A antibody, 0.63 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Gene Id | 5870 |
| Protein Name / Synonyms | RAB6A |
| Concentration (lot specific) | 0.63 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
RAB6 antibody, RAB6A' antibody, RAB6B antibody, RAB6A antibody, "RAB6, member RAS oncogene family antibody", Rab GTPase antibody, ras-related protein Rab-6A antibody, "RAB6A, member RAS oncogene family antibody"
Specificity
Species Reactivity
Human, Rat, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
24 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 208 of RAB6A (Uniprot ID# P20340)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using RAB6A(107-10931) antibody (Green) at 1:1000 dilution. Alpha-tubulin filaments were labeled at 1:2000.
RAB6A antibody detects RAB6A protein by western blot analysis.
A. 50 µg mouse brain lysate/extract
12 % SDS-PAGE
RAB6A antibody (107-10931) dilution: 1:1000
A. 50 µg mouse brain lysate/extract
12 % SDS-PAGE
RAB6A antibody (107-10931) dilution: 1:1000
RAB6A antibody detects RAB6A protein by western blot analysis.
A. 50 µg rat brain cell lysate/extract
12 % SDS-PAGE
RAB6A antibody (107-10931) dilution: 1:1000
A. 50 µg rat brain cell lysate/extract
12 % SDS-PAGE
RAB6A antibody (107-10931) dilution: 1:1000
Immunohistochemical analysis of paraffin-embedded MDA-MB-468 xenograft, using RAB6A(107-10931) antibody at 1:500 dilution.
Sample (30 ug of whole cell lysate)
A: H1299
12% SDS PAGE
107-10931 diluted at 1:1000
A: H1299
12% SDS PAGE
107-10931 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.