RAD18 antibody [N1C1], 0.46 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 56852 |
| Protein Name / Synonyms | RAD18 |
| Concentration (lot specific) | 0.46 mg/ml |
| Recommended Applications | IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
RNF73 antibody, RAD18 antibody, RING finger protein 73 antibody, postreplication repair protein hRAD18p antibody, hHR18 antibody, "RAD18, S. cerevisiae, homolog antibody", E3 ubiquitin-protein ligase RAD18 antibody, postreplication repair protein RAD18 antibody, hRAD18 antibody, RAD18 homolog (S. cerevisiae) antibody
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
56 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 259 of RAD18 (Uniprot ID# Q9NS91)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
RAD18 antibody [N1C1] detects RAD18 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RAD18 protein stained by RAD18 antibody [N1C1] (107-11103) diluted at 1:500.
Blue: Hoechst 33342 staining.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RAD18 protein stained by RAD18 antibody [N1C1] (107-11103) diluted at 1:500.
Blue: Hoechst 33342 staining.
Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using RAD18(107-11103) antibody at 1:100 dilution.
Sample (30 ug of whole cell lysate)
A: Hep G2
7.5% SDS PAGE
107-11103 diluted at 1:1000
A: Hep G2
7.5% SDS PAGE
107-11103 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.