Synonyms
DFNB24 antibody, RDX antibody, radixin antibody
Specificity
Species Reactivity
Human, Rat, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
69 kDa
Immunogen
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 95 and 434 (P35241) of Radixin
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:1000-1:10000, IHC-P: 1:100-1:1000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunohistochemical analysis of paraffin-embedded U373 xenograft, using Radixin(107-10529) antibody at 1:500 dilution.

Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using Radixin(107-10529) antibody at 1:500 dilution.

Sample (30 ug of whole cell lysate)
A: 293T
7.5% SDS PAGE
107-10529 diluted at 1:5000

Radixin antibody detects RDX protein by Western blot analysis.
A. 50 µg mouse liver lysate/extract
7.5 % SDS-PAGE
Radixin antibody (107-10529) dilution: 1:1000

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Radixin(107-10529) antibody at 1:200 dilution.
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.