RAG2 antibody, 0.37 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Rat |
| Host Species | Rabbit |
| Gene Id | 5897 |
| Protein Name / Synonyms | RAG2 |
| Concentration (lot specific) | 0.37 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
RAG-2 antibody, RAG2 antibody, V(D)J recombination-activating protein 2 antibody, recombination activating gene 2 antibody
Specificity
Species Reactivity
Human, Rat
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
59 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 271 and 527 of RAG2 (Uniprot ID# P55895)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
RAG2 antibody detects RAG2 protein by Western blot analysis.
A. 50 µg Rat thymus lysate/extract
7.5 % SDS-PAGE
RAG2 antibody (107-10861) dilution: 1:1000
A. 50 µg Rat thymus lysate/extract
7.5 % SDS-PAGE
RAG2 antibody (107-10861) dilution: 1:1000
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RAG2(107-10861) antibody at 1:200 dilution.
Immunohistochemical analysis of paraffin-embedded DLD1 Xenograft, using RAG2(107-10861) antibody at 1:100 dilution.
Sample (30 ug of whole cell lysate)
A: Molt-4
7.5% SDS PAGE
107-10861 diluted at 1:1000
A: Molt-4
7.5% SDS PAGE
107-10861 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.