RASGRP4 antibody [N1N2], N-Terminus, 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 115727 |
| Protein Name / Synonyms | RASGRP4, N-term |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
RASGRP4 antibody, RAS guanyl-releasing protein 4 antibody, guanyl nucleotide releasing protein 4 antibody, RAS guanyl releasing protein 4 antibody
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
75 kDa
Immunogen
Synthetic peptide corresponding to a region within amino acids 108 and 200 of RASGRP4 (Uniprot ID# Q8TDF6)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunofluorescence analysis of methanol-fixed HeLa, using RASGRP4(107-10475) antibody at 1:500 dilution.
Sample (30 ug of whole cell lysate)
A: Jurkat
7.5% SDS PAGE
107-10475 diluted at 1:1000
A: Jurkat
7.5% SDS PAGE
107-10475 diluted at 1:1000
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using RASGRP4 (107-10475) antibody at 1:100 dilution.
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.