Rabbit Anti-Human RCAN1 (N-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P53805 |
| Gene Id | 1827 |
| Gene Symbols | RCAN1 |
| Protein Name / Synonyms | RCAN1 (N-term) |
| Clonality | Polyclonal |
| Preparation | This RCAN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 41-68 amino acids from the N-terminal region of human RCAN1. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | Western Blotting, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
The protein encoded by this gene interacts with calcineurin A and inhibits calcineurin-dependent signaling pathways, possibly affecting central nervous system development. This gene is located in the minimal candidate region for the Down syndrome phenotype, and is overexpressed in the brain of Down syndrome fetuses. Chronic overexpression of this gene may lead to neurofibrillary tangles such as those associated with Alzheimer disease. Three transcript variants encoding three different isoforms have been found for this gene.
Specificity
Predicted Reactivity
Mouse
Other Names
RCAN1; ADAPT78; CSP1; DSC1; DSCR1; Calcipressin-1; Adapt78; Down syndrome critical region protein 1; Myocyte-enriched calcineurin-interacting protein 1; Regulator of calcineurin 1
NCBI Accession #
NP_004405.3;NP_981962.1;NP_981963.1
Other Accession #
Q9JHG6; NP_004405.3; NP_981963.1
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
28079
Recommended Dilutions
WB: 1:1000; IF: 1:10-50
 | RCAN1 Antibody (N-term) (Cat# 102-13137) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the RCAN1 antibody detected the RCAN1 protein (arrow). |
 | Western blot analysis of RCAN1 (arrow) using rabbit polyclonal RCAN1 Antibody (N-term) (Cat# 102-13137). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RCAN1 gene. |
 | Fluorescent confocal image of A431 cell stained with RCAN1 Antibody (N-term)(Cat# 102-13137).A431 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RCAN1 primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). RCAN1 immunoreactivity is localized to Cytoplasm significantly. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.