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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P35398 |
| Gene Id | 6095 |
| Gene Symbols | RORA |
| Protein Name / Synonyms | RORA (T216) |
| Clonality | Polyclonal |
| Preparation | This RORA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 193-222 amino acids from human RORA. |
| Concentration (lot specific) | 0.360 mg/ml |
| Recommended Applications | FC, Western Blotting, IF |
| Research Area | Angiogenesis, Cancer, Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
RORA is a member of the NR1 subfamily of nuclear hormone receptors. It can bind as a monomer or as a homodimer to hormone response elements upstream of several genes to enhance the expression of those genes. The specific functions of this protein are not known, but it has been shown to interact with NM23-2, a nucleoside diphosphate kinase involved in organogenesis and differentiation, as well as with NM23-1, the product of a tumor metastasis suppressor candidate gene.
Specificity
Predicted Reactivity
Mouse
Other Names
RORA; NR1F1; RZRA; Nuclear receptor ROR-alpha; Nuclear receptor RZR-alpha; Nuclear receptor subfamily 1 group F member 1; Retinoid-related orphan receptor-alpha
NCBI Accession #
NP_002934.1;NP_599022.1;NP_599023.1;NP_599024.1
Other Accession #
P51448; NP_599022
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
63036
Recommended Dilutions
WB: 1:1000; IF: 1:10-50
 | Western blot analysis of anti-RORA Antibody (T216) (Cat# 102-10828) in A2058 cell line lysates (35ug/lane). RORA (arrow) was detected using the purified Pab. |
 | Fluorescent confocal image of Hela cell stained with RORA Antibody (T216)(Cat# 102-10828).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RORA primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). RORA immunoreactivity is localized to Nucleus significantly. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.
