Rabbit Anti-Human RORA (T216)

Rabbit Anti-Human RORA (T216) Antibody, 400 ul
$334.00
In stock
Code:
102-10828
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Product Description

Specifications

Size 400 µl
Protein Name / Synonyms RORA (T216)
Accession Number P35398
Gene Symbols RORA
Gene Id 6095
Concentration (lot specific) 0.360 mg/ml
Preparation This RORA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 193-222 amino acids from human RORA.
Host Species Rabbit
Clonality Polyclonal
Species Human

Introduction

RORA is a member of the NR1 subfamily of nuclear hormone receptors. It can bind as a monomer or as a homodimer to hormone response elements upstream of several genes to enhance the expression of those genes. The specific functions of this protein are not known, but it has been shown to interact with NM23-2, a nucleoside diphosphate kinase involved in organogenesis and differentiation, as well as with NM23-1, the product of a tumor metastasis suppressor candidate gene.

Specificity

Predicted Reactivity
Mouse
Other Names
RORA; NR1F1; RZRA; Nuclear receptor ROR-alpha; Nuclear receptor RZR-alpha; Nuclear receptor subfamily 1 group F member 1; Retinoid-related orphan receptor-alpha
NCBI Accession #
NP_002934.1;NP_599022.1;NP_599023.1;NP_599024.1
Other Accession #
P51448; NP_599022

Format

Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
63036
Recommended Dilutions
WB: 1:1000; IF: 1:10-50
Western blot analysis of anti-RORA Antibody (T216) (Cat# 102-10828) in A2058 cell line lysates (35ug/lane). RORA (arrow) was detected using the purified Pab.
Fluorescent confocal image of Hela cell stained with RORA Antibody (T216)(Cat# 102-10828).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RORA primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). RORA immunoreactivity is localized to Nucleus significantly.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.
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