RPA70 antibody [C1C3], 0.49 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 6117 |
| Protein Name / Synonyms | RPA70 |
| Concentration (lot specific) | 0.49 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
HSSB antibody, MST075 antibody, REPA1 antibody, RF-A antibody, RP-A antibody, RPA70 antibody, RPA1 antibody, single-stranded DNA-binding protein antibody, replication protein A 70 kDa DNA-binding subunit antibody, replication factor A protein 1 antibody, RP-A p70 antibody, RF-A protein 1 antibody, MSTP075 antibody, "replication protein A1, 70kDa antibody"
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
68 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 369 and 616 of RPA70 (Uniprot ID# P27694)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Sample (50 ug of whole cell lysate)
A: mouse liver
7.5% SDS PAGE
107-10795 diluted at 1:1000
A: mouse liver
7.5% SDS PAGE
107-10795 diluted at 1:1000
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RPA 70 kDa subunit (107-10795) antibody at 1:200 dilution.
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using RPA 70 kDa subunit (107-10795) antibody at 1:100 dilution.
Sample(30 ug whole cell lysate)
A:H1299
B:HeLa S3
7.5% SDS PAGE
107-10795 diluted at 1:1000
A:H1299
B:HeLa S3
7.5% SDS PAGE
107-10795 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.