Rabbit Anti-Human RUNX2 (S533) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q13950 |
| Gene Id | 860 |
| Gene Symbols | RUNX2, OSF2, AML3, CBFA1, PEBP2A |
| Protein Name / Synonyms | Runt-related transcription factor 2, Acutemyeloid leukemia 3 protein, Core-bindingfactor subunit alpha-1, CBF-alpha-1,Oncogene AML-3, Osteoblast-specific transcription factor 2, OSF-2, Polyomavirusenhancer-binding protein 2 alpha A subunit, PEA2-alpha A, PEBP2-alpha A, SL3-3 enhancer factor 1 alpha A subunit, SL3/AKVcore-binding factor alpha A subunit, RUNX2,AML3, CBFA1, OSF2, PEBP2A |
| Clonality | Polyclonal |
| Preparation | This RUNX2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 445-474 amino acids surrounding S533 of human RUNX2. |
| Isotype | Kappa, Rabbit |
| Concentration (lot specific) | 0.3 mg/ml |
| Buffers | PBS with 0.09% (W/V) sodium azide |
| Recommended Applications | Western Blotting, IHC-P, FC |
| Domain / Region / Terminus | 445-474 |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
Runx2 is a member of the RUNX family of transcription factors. It is a nuclear protein with an Runt DNA-binding domain. This protein is essential for osteoblastic differentiation and skeletal morphogenesis and acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. It can bind DNA both as a monomer or, with more affinity, as a subunit of a heterodimeric complex. Mutations in the Runx2 gene have been associated with the bone development disorder cleidocranial dysplasia (CCD).
Specificity
NCBI Accession #
NP_001015051.3;NP_001019801.3
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
56648
Recommended Dilutions
WB: 1:1000; IHC: 1:10-50; FC: 1:10-50
 | RUNX2 Antibody (S533) (Cat# 102-17593) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the RUNX2 antibody detected the RUNX2 protein (arrow). |
 | RUNX2 Antibody (S533) (102-17593)immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RUNX2 Antibody (S533) for immunohistochemistry. Clinical relevance has not been evaluated. |
 | RUNX2 Antibody (S533) (Cat# 102-17593) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.