Synonyms
MLN70 antibody, S100C antibody, S100A11 antibody, protein S100-A11 antibody, S100 calcium-binding protein A11 (calgizzarin) antibody, metastatic lymph node gene 70 protein antibody, calgizzarin antibody, protein S100-C antibody, MLN 70 antibody, S100 calcium binding protein A11 antibody
Specificity
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
12 kDa
Immunogen
Synthetic peptide corresponding to a region within amino acids 8 and 102 of S100A11 (Uniprot ID# P31949)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

S100A11 antibody detects S100A11 protein by Western blot analysis.
A. 30 µg A431 whole cell lysate/extract
15 % SDS-PAGE
S100A11 antibody (107-10236) dilution: 1:1000

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using S100A11(107-10236) antibody at 1:200 dilution.

Immunohistochemical analysis of paraffin-embedded SW480 Xenograft, using S100A11(107-10236) antibody at 1:500 dilution.
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.