Rabbit Anti-Human S6K (RPS6KB1) (Center) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P23443 |
| Gene Id | 6198 |
| Gene Symbols | RPS6KB1 |
| Protein Name / Synonyms | S6K (RPS6KB1) (Center) |
| Clonality | Polyclonal |
| Preparation | This S6K (RPS6KB1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 277-306 amino acids from the Central region of human S6K (RPS6KB1). |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | Western Blotting, IHC-P |
| Research Area | mTOR Signaling |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates several residues of the S6 ribosomal protein. The kinase activity of this protein leads to an increase in protein synthesis and cell proliferation. Amplification of the region of DNA encoding the gene for RPS6KB1 and overexpression of this kinase are seen in some breast cancer cell lines.
Specificity
Predicted Reactivity
Mouse, Rat, Bovine, Rabbit
Other Names
RPS6KB1; STK14A; Ribosomal protein S6 kinase beta-1; 70 kDa ribosomal protein S6 kinase 1; Ribosomal protein S6 kinase I; Serine/threonine-protein kinase 14A; p70 ribosomal S6 kinase alpha
NCBI Accession #
NP_001258989.1;NP_003152.1
Other Accession #
P67999; P67998; Q8BSK8; Q6TJY3
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
59140
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100
 | Western blot analysis of anti-RPS6KB1 Antibody (Center) Pab (Cat# 102-15369) in Jurkat cell line lysates (35ug/lane). RPS6KB1 Antibody (Center))(arrow) was detected using the purified Pab. |
 | Western blot analysis of anti-RPS6KB1 Antibody (Center) Pab (Cat# 102-15369) pre-incubated with and without blocking peptide (BP#NULL) in 293 cell line lysate. RPS6KB1 Antibody (Center)(arrow) was detected using the purified Pab. |
 | Western blot analysis of anti-RPS6KB1 Antibody (Center) Pab (Cat# 102-15369) in 293 cell line lysates transiently transfected with the RPS6KB1 gene (2ug/lane). RPS6KB1(arrow) was detected using the purified Pab. |
 | Formalin-fixed and paraffin-embedded human breast carcinoma reacted with RPS6KB1 Antibody (Center)(Cat# 102-15369), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.