Synonyms
DKFZp779C1727 antibody, HAP antibody, HET antibody, SAF-B1 antibody, SAFB1 antibody, SAFB antibody, glutathione S-transferase fusion protein antibody, HSP27 estrogen response element-TATA box-binding protein antibody, SAF-B antibody, SAB-B1 antibody, Hsp27 ERE-TATA binding protein antibody, HSP27 ERE-TATA-binding protein antibody, scaffold attachment factor B1 antibody, heat-shock protein (HSP27) estrogen response element and TATA box-binding protein antibody, scaffold attachment factor B antibody
Specificity
Species Reactivity
Human, Rat, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
103 kDa
Immunogen
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 476 and 695 (Q15424) of SAFB
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000, IHC-P: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Scaffold attachment factor B1 antibody detects Scaffold attachment factor B1 protein at nucleus on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
Scaffold attachment factor B1 antibody (107-10260) dilution: 1:500.

Scaffold attachment factor B1 antibody detects Scaffold attachment factor B1 protein at nucleus on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse esophagus.
Scaffold attachment factor B1 antibody (107-10260) dilution: 1:500.

Scaffold attachment factor B1 antibody detects Scaffold attachment factor B1 protein at nucleus on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Scaffold attachment factor B1 antibody (107-10260) dilution: 1:500.

Scaffold attachment factor B1 antibody detects Scaffold attachment factor B1 protein at nucleus on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
Scaffold attachment factor B1 antibody (107-10260) dilution: 1:500.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Scaffold attachment factor B1(107-10260) antibody at 1:100 dilution.

Sample (30 ug of whole cell lysate)
A: A431
B: Hep G2
7.5% SDS PAGE
107-10260 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.