SCARA3 antibody [N2C2], Internal, 0.78 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 51435 |
| Protein Name / Synonyms | SCARA3, Internal |
| Concentration (lot specific) | 0.78 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
APC7 antibody, CSR antibody, CSR1 antibody, MSLR1 antibody, MSRL1 antibody, SCARA3 antibody, macrophage scavenger receptor-like 1 antibody, cellular stress response protein antibody, cellular stress response gene protein antibody, scavenger receptor class A member 3 antibody, "scavenger receptor class A, member 3 antibody"
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
65 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 187 and 474 of SCARA3 (Uniprot ID# Q6AZY7)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000[suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
SCARA3 antibody [N2C2], Internal detects SCARA3 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in -20? 100% MeOH for 5 min.
Green: SCARA3 protein stained by SCARA3 antibody [N2C2], Internal (107-10233) diluted at 1:500.
Blue: Hoechst 33343 staining.
Sample: HeLa cells were fixed in -20? 100% MeOH for 5 min.
Green: SCARA3 protein stained by SCARA3 antibody [N2C2], Internal (107-10233) diluted at 1:500.
Blue: Hoechst 33343 staining.
Immunohistochemical analysis of paraffin-embedded CL1-0 xenograft, using SCARA3(107-10233) antibody at 1:100 dilution.
Sample(30 µg of whole cell lysate)
A:Hep G2
7.5% SDS PAGE
107-10233 diluted at 1:1000
A:Hep G2
7.5% SDS PAGE
107-10233 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.