Rabbit Anti-Human SCF (KITLG) (C-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P21583 |
| Gene Id | 4254 |
| Gene Symbols | KITLG |
| Protein Name / Synonyms | SCF (KITLG) (C-term) |
| Clonality | Polyclonal |
| Preparation | This SCF (KITLG) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 244-273 amino acids from the C-terminal region of human SCF (KITLG). |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | Western Blotting, IF, FC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
KITLG is the ligand of the tyrosine-kinase receptor encoded by the KIT locus. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development, and hematopoiesis, all believed to reflect a role in cell migration. In adults, it functions pleiotropically, while mostly noted for its continued requirement in hematopoiesis.
Specificity
Predicted Reactivity
Bovine, Pig
Other Names
KITLG; MGF; SCF; Kit ligand; Mast cell growth factor; Stem cell factor; c-Kit ligand; Soluble KIT ligand
NCBI Accession #
NP_000890.1;NP_003985.2
Other Accession #
Q29030; Q28132
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
30899
Recommended Dilutions
WB: 1:1000; IF: 1:10-50; FC: 1:10-50
 | Western blot analysis of KITLG Antibody (C-term) (Cat# 102-12685) in 293 cell line lysates (35ug/lane). KITLG (arrow) was detected using the purified Pab. |
 | Western blot analysis of KITLG(arrow) using rabbit polyclonal KITLG Antibody (C-term) (Cat# 102-12685). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the KITLG gene (Lane 2) (Origene Technologies). |
 | Immunofluorescence analysis of anti-KITLG Antibody (C-term) (Cat# 102-12685) in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI. |
 | Flow cytometric analysis of WiDr cells using SCF (KITLG) Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.