SEC61A1 antibody, 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 29927 |
| Protein Name / Synonyms | SEC61A1 |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
HSEC61 antibody, SEC61 antibody, SEC61A antibody, SEC61A1 antibody, protein transport protein SEC61 alpha subunit antibody, sec61 homolog antibody, Sec61 alpha-1 antibody, protein transport protein Sec61 subunit alpha antibody, protein transport protein Sec61 subunit alpha isoform 1 antibody, Sec61 alpha 1 subunit (S. cerevisiae) antibody
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
52 kDa
Immunogen
Synthetic peptide corresponding to a region within amino acids 413 and 476 of SEC61A1
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunofluorescence analysis of methanol-fixed A431, using SEC61A1(107-10625) antibody at 1:200 dilution.
Sample (30 ug of whole cell lysate)
A: K562
10% SDS PAGE
107-10625 diluted at 1:1000
A: K562
10% SDS PAGE
107-10625 diluted at 1:1000
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using SEC61A1(107-10625) antibody at 1:500 dilution.
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.