Synonyms
MGC4980 antibody, SELD antibody, SPS antibody, SPS1 antibody, SEPHS1 antibody, selenophosphate synthetase 1 delta E2 antibody, selenium donor protein 1 antibody, selenophosphate synthetase 1 +E9a antibody, selenophosphate synthetase 1 major antibody, selenophosphate synthetase 1 +E9 antibody, selenophosphate synthetase 1 delta E8 antibody, selenophosphate synthase 1 antibody, selenide, water dikinase 1 antibody, selenophosphate synthetase 1 antibody
Specificity
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
43 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 204 of Selenophosphate synthetase 1 (Uniprot ID# P49903)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Selenophosphate synthetase 1 antibody detects SEPHS1 protein at nucleus on Colon Carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded Colon Carcinoma.
Selenophosphate synthetase 1 antibody (107-10685) dilution: 1:500.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Selenophosphate synthetase 1(107-10685) antibody at 1:200 dilution.

Sample(30 µg of whole cell lysate)
A:A431
B:MOLT4
12% SDS PAGE
107-10685 diluted at 1:500
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.