Synonyms
2PP2A antibody, I2PP2A antibody, IGAAD antibody, IPP2A2 antibody, PHAPII antibody, TAF-I antibody, TAF-IBETA antibody, SET antibody, inhibitor-2 of protein phosphatase-2A antibody, phosphatase 2A inhibitor I2PP2A antibody, inhibitor of granzyme A-activated DNase antibody, HLA-DR-associated protein II antibody, SET translocation (myeloid leukemia-associated) antibody, "Template-Activating Factor-I, chromatin remodelling factor antibody", protein SET antibody, protein phosphatase type 2A inhibitor antibody, SET nuclear oncogene antibody
Specificity
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
33 kDa
Immunogen
Synthetic peptide corresponding to a region within amino acids 1 and 45 of SET
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections)
Application Notes
Suggested starting dilutions are as follows: IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using SET antibody, isoform 2-specifc (107-10612) at 1:100 dilution.

SET antibody, isoform 2-specifc detects SET protein by western blot analysis.
A. 30 µg 293T whole cell lysate/extract
B. 30 µg whole cell lysate/extract of human SET-transfected 293T cells
12 % SDS-PAGE
SET antibody, isoform 2-specifc (107-10612) dilution: 1:1000

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using SET antibody, isoform 2-specifc (107-10612) at 1:200 dilution.
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.