Synonyms
HSS antibody, MPS3A antibody, SFMD antibody, SGSH antibody, sulfoglucosamine sulfamidase antibody, sulphamidase antibody, mucopolysaccharidosis type IIIA antibody, heparan sulfate sulfatase antibody, N-sulphoglucosamine sulphohydrolase antibody, N-sulfoglucosamine sulfohydrolase antibody
Specificity
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
57 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 318 and 496 of SGSH (Uniprot ID# P51688)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:1000-1:10000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft , using SGSH(107-10277) antibody at 1:100 dilution.

Sample (30 ug of whole cell lysate)
A: Molt-4
7.5% SDS PAGE
107-10277 diluted at 1:5000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.