Synonyms
SGT antibody, alphaSGT antibody, hSGT antibody, SGTA antibody, protein containing three tetratricopeptide repeats antibody, vpu-binding protein antibody, small glutamine-rich tetratricopeptide repeat-containing protein alpha antibody, UBP antibody, alpha-SGT antibody, small glutamine-rich tetratricopeptide repeat (TPR)-containing, alpha antibody
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.01%thimerosal in PBS
Calculated Molecular Weight
34 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 6 and 313 of SGTA (Uniprot ID# O43765)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:1000-1:10000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

SGTA antibody detects SGTA protein at cytosol on Lung adenocarcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded Lung adenocarcinoma.
SGTA antibody (107-10268) dilution: 1:500.

Sample (50 ug of whole cell lysate)
A: mouse liver
10% SDS PAGE
107-10268 diluted at 1:1000

Sample (30 ug of whole cell lysate)
A: Hep G2
B: Molt-4
10% SDS PAGE
107-10268 diluted at 1:5000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.