Rabbit Anti-Human SIX5 (Center) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q8N196 |
| Gene Id | 147912 |
| Gene Symbols | SIX5 |
| Protein Name / Synonyms | SIX5 (Center) |
| Clonality | Polyclonal |
| Preparation | This SIX5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 257-286 amino acids from the Central region of human SIX5. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
The protein encoded by this gene is a homeodomain-containing transcription factor that appears to function in the regulation of organogenesis. This gene is located downstream of the dystrophia myotonica-protein kinase gene. Mutations in this gene are a cause of branchiootorenal syndrome type 2.
Specificity
Predicted Reactivity
Mouse
Other Names
SIX5; DMAHP; Homeobox protein SIX5; DM locus-associated homeodomain protein; Sine oculis homeobox homolog 5
NCBI Accession #
NP_787071.2
Other Accession #
P70178; NP_787071.2
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
74562
Recommended Dilutions
WB: 1:1000; IHC: 1:10-50; IF: 1:10-50
 | SIX5 Antibody (Center) (Cat# 102-11710) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the SIX5 antibody detected the SIX5 protein (arrow). |
 | SIX5 Antibody (Center) (Cat# 102-11710)immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SIX5 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated. |
 | Fluorescent confocal image of Hela cell stained with SIX5 Antibody (Center)(Cat# 102-11710).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SIX5 primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). SIX5 immunoreactivity is localized to Cytoplasm significantly. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.