Rabbit Anti-Human SMAD3 (Center) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P84022 |
| Gene Id | 4088 |
| Gene Symbols | SMAD3 |
| Protein Name / Synonyms | SMAD3 (Center) |
| Clonality | Polyclonal |
| Preparation | This SMAD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 127-156 amino acids from the Central region of human SMAD3. |
| Concentration (lot specific) | 0.250 mg/ml |
| Recommended Applications | Western Blotting, IF, IHC-P |
| Research Area | Cardiovascular Disease, Cancer, Inflammation, TGF-beta Signaling |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.
Specificity
Predicted Reactivity
Mouse, Rat, Chicken, Pig
Other Names
SMAD3; MADH3; Mothers against decapentaplegic homolog 3; JV15-2; SMAD family member 3
NCBI Accession #
NP_001138574.1;NP_001138575.1;NP_001138576.1;NP_005893.1
Other Accession #
P84025; P84024; Q8BUN5; P84023; NP_005893.1; NP_001138574.1
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
48081
Recommended Dilutions
WB: 1:1000; IF: 1:10-50; IHC: 1:10-50
 | SMAD3 Antibody (Center) (Cat# 102-12047) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the SMAD3 antibody detected the SMAD3 protein (arrow). |
 | Confocal immunofluorescent analysis of SMAD3 Antibody (Center)(Cat# 102-12047) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue). |
 | SMAD3 Antibody (Center) (Cat# 102-12047)immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SMAD3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.