Rabbit Anti-Human SMAD4 (T277) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q13485 |
| Gene Id | 4089 |
| Gene Symbols | SMAD4 |
| Protein Name / Synonyms | SMAD4 (T277) |
| Clonality | Polyclonal |
| Preparation | This SMAD4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 255-284 amino acids from human SMAD4. |
| Concentration (lot specific) | 0.390 mg/ml |
| Recommended Applications | Western Blotting, IF |
| Research Area | Cardiovascular Disease, Cancer, TGF-beta Signaling |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
SMAD4 is the common SMAD (co-SMAD)mediator of signal transduction by TGF-beta (transforming growth factor). It promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. It may act as a tumor suppressor.
Specificity
Predicted Reactivity
Mouse, Rat
Other Names
SMAD4; DPC4; MADH4; Mothers against decapentaplegic homolog 4; Deletion target in pancreatic carcinoma 4; SMAD family member 4
NCBI Accession #
NP_005350.1
Other Accession #
O70437; P97471
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
60439
Recommended Dilutions
WB: 1:1000; IF: 1:10-50
 | Western blot analysis of SMAD4 Antibody (T277)(arrow) using rabbit polyclonal SMAD4 Antibody (T277) (Cat# 102-17602). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SMAD4 gene (Lane 2) (Origene Technologies). |
 | Confocal immunofluorescent analysis of SMAD4 Antibody (T277)(Cat# 102-17602) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue). |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.