Synonyms
MADH6 antibody, MADH9 antibody, SMAD8 antibody, SMAD8A antibody, SMAD8B antibody, SMAD9 antibody, "Mothers against decapentaplegic, drosophila, homolog of, 9 antibody", "SMAD, mothers against DPP homolog 9 antibody", MAD homolog 9 antibody, mothers against decapentaplegic homolog 9 antibody, SMAD family member 9 antibody
Specificity
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
52 kDa
Immunogen
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 166 and 430 of SMAD9
Applications
Applications Tested
ICC/IF,Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using SMAD9(107-11127) antibody at 1:500 dilution.

Sample (30 ug of whole cell lysate)
A: NT2D1
10% SDS PAGE
107-11127 diluted at 1:500
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.