SMC1B antibody [N1N3], 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 27127 |
| Protein Name / Synonyms | SMC1B |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
FLJ43748 antibody, SMC1BETA antibody, SMC1L2 antibody, bK268H5 antibody, bK268H5.5 antibody, SMC1B antibody, SMC-1B antibody, "SMC1 (structural maintenance of chromosomes 1, yeast)-like 1 antibody", SMC protein 1B antibody, mitosis-specific chromosome segregation protein like protein beta antibody, SMC-1-beta antibody, structural maintenance of chromosomes protein 1B antibody, SMC1 structural maintenance of chromosomes 1-like 2 antibody, structural maintenance of chromosomes 1B antibody
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
144 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 154 and 433 of SMC1B (Uniprot ID# Q8NDV3)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunofluorescence analysis of paraformaldehyde-fixed A431, using SMC1B(107-10997) antibody at 1:500 dilution.
Immunohistochemical analysis of paraffin-embedded SAS xenograft, using SMC1B(107-10997) antibody at 1:500 dilution.
Sample (30 ug of whole cell lysate)
A: Hela
5% SDS PAGE
107-10997 diluted at 1:1000
A: Hela
5% SDS PAGE
107-10997 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.