Synonyms
CIS6 antibody, CISH6 antibody, Cish5 antibody, KIAA0671 antibody, SOCS-5 antibody, SOCS5 antibody, CIS-6 antibody, cytokine-inducible SH2-containing protein 5 antibody, suppressor of cytokine signaling 5 antibody, cytokine-inducible SH2 protein 6 antibody
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
61 kDa
Immunogen
Synthetic peptide corresponding to a region within amino acids 1 and 42 of SOCS5
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunohistochemical analysis of paraffin-embedded Ca922 xenograft, using SOCS5(107-10478) antibody at 1:100 dilution.

SOCS5 antibody detects SOCS5 protein by Western blot analysis.
A. 30 µg NT2D1 whole cell lysate/extract
B. 30 µg PC-3 whole cell lysate/extract
7.5 % SDS-PAGE
SOCS5 antibody (107-10478) dilution: 1:500

SOCS5 antibody detects SOCS5 protein by Western blot analysis.
A. 50 µg mouse testis lysate/extract
7.5 % SDS-PAGE
SOCS5 antibody (107-10478) dilution: 1:500

Sample (30 ug of whole cell lysate)
A: Molt-4
7.5% SDS PAGE
SOCS5 antibody
107-10478 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.