Rabbit Anti-Human SOD1 (Center) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P00441 |
| Gene Id | 6647 |
| Gene Symbols | SOD1 |
| Protein Name / Synonyms | SOD1 (Center) |
| Clonality | Polyclonal |
| Preparation | This SOD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 55-84 amino acids from the Central region of human SOD1. |
| Concentration (lot specific) | 0.367 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, IF, FC |
| Research Area | Cardiovascular Disease, Inflammation, Oxidative Stress |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
SOD1 binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. This isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein.
Specificity
Other Names
SOD1; Superoxide dismutase [Cu-Zn]; Superoxide dismutase 1
NCBI Accession #
NP_000445.1
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
15936
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100; FC: 1:10-50; IF: 1:10-50
 | Western blot analysis of SOD1 Antibody (Center) (Cat# 102-18006) in Jurkat cell line lysates (35ug/lane). SOD1 (arrow) was detected using the purified Pab. |
 | Formalin-fixed and paraffin-embedded human colon carcinoma reacted with SOD1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
 | SOD1 Antibody (Center) (Cat# 102-18006) flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
 | Confocal immunofluorescent analysis of SOD1 Antibody (Center) (Cat# 102-18006) with 293 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue). |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.