Rabbit Anti-Human SOX2 (N-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P48431 |
| Gene Id | 6657 |
| Gene Symbols | SOX2 |
| Protein Name / Synonyms | SOX2 (N-term) |
| Clonality | Polyclonal |
| Preparation | This SOX2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-119 amino acids from the N-terminal region of human SOX2. |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, IF, FC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
The intronless gene for SOX2 encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The encoded protein may act as a transcriptional activator after forming a protein complex with other proteins. Mutations in the SOX2 gene have been associated with bilateral anophthalmia, a severe form of structural eye malformation.
Specificity
Predicted Reactivity
Mouse, Zebrafish, Chicken, Xenopus
Other Names
SOX2; Transcription factor SOX-2
NCBI Accession #
NP_003097.1
Other Accession #
Q6EJB7; O42569; P48432; Q6P0E1; P48430; Q2PG84; P53783; O00570; O57401; Q2Z1R2; Q6DGL6
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
34310
Recommended Dilutions
WB: 1:1000; IHC: 1:10-50; IF: 1:10-50; FC: 1:10-50
 | Western blot analysis of SOX2 Antibody (N-term) in 293 cell line lysates (35ug/lane). SOX2(arrow) was detected using the purified Pab. |
 | Western blot analysis of SOX2 (arrow) using rabbit polyclonal SOX2 Antibody (N-term) (Cat# 102-14836). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SOX2 gene (Lane 2) (Origene Technologies). |
 | Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with SOX2 antibody (N-term) (Cat# 102-14836), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
 | Immunofluorescence analysis of SOX2 Antibody (N-term) (Cat# 102-14836) in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI. |
 | Flow cytometric analysis of NCI-H292 cells using SOX2 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.
