Rabbit Anti-Human SP1 (C-term P692) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P08047 |
| Gene Id | 6667 |
| Gene Symbols | SP1 |
| Protein Name / Synonyms | SP1 (C-term P692) |
| Clonality | Polyclonal |
| Preparation | This SP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 677-707 amino acids from the C-terminal region of human SP1. |
| Concentration (lot specific) | 0.450 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, FC, IF |
| Research Area | Angiogenesis |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays an essential role in the regulation of FE65 gene expression.
Specificity
Predicted Reactivity
Mouse, Rat
Other Names
SP1; TSFP1; Transcription factor Sp1
NCBI Accession #
NP_001238754.1;NP_003100.1;NP_612482.2
Other Accession #
Q01714; O89090; NP_612482.2
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
80693
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100; FC: 1:10-50; IF: 1:10-50
 | SP1 Antibody (C-term P692) (Cat# 102-11100) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the SP1 antibody detected the SP1 protein (arrow). |
 | SP1 Antibody (C-term P692) (Cat# 102-11100)immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SP1 Antibody (C-term P692) for immunohistochemistry. Clinical relevance has not been evaluated. |
 | SP1 Antibody (C-term P692) (Cat# 102-11100) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
 | Confocal immunofluorescent analysis of SP1 Antibody (C-term P692)(Cat# 102-11100) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue). |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.