SPFH2 antibody [C2C3], C-Terminus, 0.41 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 11160 |
| Protein Name / Synonyms | SPFH2, C-term |
| Concentration (lot specific) | 0.41 mg/ml |
| Recommended Applications | IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
C8orf2 antibody, Erlin-2 antibody, MGC87072 antibody, NET32 antibody, SPFH2 antibody, ERLIN2 antibody, "SPFH domain family, member 2 antibody", stomatin-prohibitin-flotillin-HflC/K domain-containing protein 2 antibody, endoplasmic reticulum lipid raft-associated protein 2 antibody, SPFH domain-containing protein 2 antibody, ER lipid raft associated 2 antibody
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
38 kDa
Immunogen
Synthetic peptide corresponding to a region within amino acids 278 and 339 of SPFH2 (Uniprot ID# O94905)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunohistochemical analysis of paraffin-embedded endo mitral OVCA, using ERLIN2(107-10599) antibody at 1:100 dilution.
Sample(30 µg of whole cell lysate)
A:HeLa S3
12% SDS PAGE
107-10599 diluted at 1:500
A:HeLa S3
12% SDS PAGE
107-10599 diluted at 1:500
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.