Rabbit Anti-Human SSH3 (C-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q8TE77 |
| Gene Id | 54961 |
| Gene Symbols | SSH3 |
| Protein Name / Synonyms | SSH3 (C-term) |
| Clonality | Polyclonal |
| Preparation | This SSH3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 575-602 amino acids from the C-terminal region of human SSH3. |
| Concentration (lot specific) | 0.280 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, FC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
The ADF (actin-depolymerizing factor)/cofilin family (see MIM 601442) is composed of stimulus-responsive mediators of actin dynamics. ADF/cofilin proteins are inactivated by kinases such as LIM domain kinase-1 (LIMK1; MIM 601329). The SSH family appears to play a role in actin dynamics by reactivating ADF/cofilin proteins in vivo (Niwa et al., 2002 [PubMed 11832213]).
Specificity
Other Names
SSH3; SSH3L; Protein phosphatase Slingshot homolog 3; SSH-like protein 3
NCBI Accession #
NP_060327.3
Other Accession #
NP_060327.3
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
72996
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100; FC: 1:10-50; IF: 1:10-50
 | SSH3 Antibody (C-term) (Cat# 102-11213) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the SSH3 antibody detected the SSH3 protein (arrow). |
 | SSH3 Antibody (C-term) (Cat# 102-11213)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SSH3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated. |
 | SSH3 Antibody (C-term) (Cat# 102-11213) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
 | Confocal immunofluorescent analysis of SSH3 Antibody (C-term)(Cat# 102-11213) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue). |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.