Rabbit Anti-Human STAT3 (C-term S727) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P40763 |
| Gene Id | 6774 |
| Gene Symbols | STAT3 |
| Protein Name / Synonyms | STAT3 (C-term S727) |
| Clonality | Polyclonal |
| Preparation | This STAT3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide from the C-terminal region of human STAT3, aa 711-743. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | IF, Western Blotting |
| Research Area | Angiogenesis, Cancer, Inflammation, Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Three alternatively spliced transcript variants encoding distinct isoforms have been described.
Specificity
Other Names
STAT3; APRF; Signal transducer and activator of transcription 3; Acute-phase response factor
NCBI Accession #
NP_003141.2;NP_644805.1;NP_998827.1
Other Accession #
NP_003141.2
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
88068
Recommended Dilutions
WB: 1:1000; IF: 1:200
 | STAT3 Antibody (pS727) (Cat# 102-13659) western blot analysis in Daudi cell line lysates (35ug/lane).This demonstrates the STAT3 antibody detected the STAT3 protein (arrow). |
 | Fluorescent confocal image of SY5Y cells stained with STAT3 (C-term S727) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with 102-13659 STAT3 (C-term S727) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 5 min). Note the highly specific localization of the STAT3 mainly to the nucleus, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000168610). |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.
