STK4 antibody, 1 mg/ml (100 ul)
Loading...
Loading...
Loading...
Loading...
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 6789 |
| Protein Name / Synonyms | STK4 |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
DKFZp686A2068 antibody, KRS2 antibody, MST1 antibody, YSK3 antibody, STK4 antibody, mammalian sterile 20-like 1 antibody, kinase responsive to stress 2 antibody, serine/threonine-protein kinase Krs-2 antibody, MST-1 antibody, "dJ211D12.2 (serine/threonine kinase 4 (MST1, KRS2)) antibody", STE20-like kinase MST1 antibody, mammalian STE20-like protein kinase 1 antibody, serine/threonine-protein kinase 4 antibody, serine/threonine kinase 4 antibody
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
56 kDa
Immunogen
Synthetic peptide corresponding to a region within amino acids 324 and 418 of STK4 (Uniprot ID# Q13043)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using STK4(107-10831) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled at 1:2000.
STK4 antibody immunoprecipitates STK4 protein in IP experiments.
IP samples: HepG2 whole cell extract
A. 50 µg HepG2 whole cell extract
B. Control with 4 µg of preimmune Rabbit IgG
C. Immunoprecipitation of STK4 protein by 4 µg STK4 antibody (107-10831)
7.5 % SDS-PAGE
The immunoprecipitated STK4 protein was detected by STK4 antibody (107-10831) diluted at 1:500.
[EasyBlot anti-rabbit IgG was used as a secondary reagent]
IP samples: HepG2 whole cell extract
A. 50 µg HepG2 whole cell extract
B. Control with 4 µg of preimmune Rabbit IgG
C. Immunoprecipitation of STK4 protein by 4 µg STK4 antibody (107-10831)
7.5 % SDS-PAGE
The immunoprecipitated STK4 protein was detected by STK4 antibody (107-10831) diluted at 1:500.
[EasyBlot anti-rabbit IgG was used as a secondary reagent]
Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
107-10831 diluted at 1:2000
A: Mouse brain
7.5% SDS PAGE
107-10831 diluted at 1:2000
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using MST1(107-10831) antibody at 1:500 dilution.
Sample (30 ug of whole cell lysate)
A: Hep G2
7.5% SDS PAGE
STK4 antibody
107-10831 diluted at 1:1000
A: Hep G2
7.5% SDS PAGE
STK4 antibody
107-10831 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.