Rabbit Anti-Human T (N-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | O15178 |
| Gene Id | 6862 |
| Gene Symbols | T |
| Protein Name / Synonyms | T (N-term) |
| Clonality | Polyclonal |
| Preparation | This T antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15-43 amino acids from the N-terminal region of human T. |
| Concentration (lot specific) | 0.390 mg/ml |
| Recommended Applications | Western Blotting, IF |
| Research Area | Cardiovascular Disease, Cancer |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells.
Specificity
Predicted Reactivity
Mouse, Chicken
Other Names
T; Brachyury protein; Protein T
NCBI Accession #
NP_001257413.1;NP_003172.1
Other Accession #
P20293; P79777; NP_003172.1
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
47443
Recommended Dilutions
WB: 1:1000; IF: 1:10-50
 | T Antibody (N-term) (Cat# 102-14585) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the T antibody detected the T protein (arrow). |
 | Fluorescent confocal image of Hela cell stained with T Antibody (N-term)(Cat# 102-14585).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with T primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). T immunoreactivity is localized to Nucleus significantly. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.