TAP antibody [N1N2], N-Terminus, 0.3 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 10482 |
| Protein Name / Synonyms | TAP, N-term |
| Concentration (lot specific) | 0.3 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
DKFZp667O0311 antibody, MEX67 antibody, TAP antibody, NXF1 antibody, tip-associated protein antibody, nuclear RNA export factor 1 antibody, mRNA export factor TAP antibody, tip-associating protein antibody, tip associating protein antibody
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
70 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 151 of TAP (Uniprot ID# Q9UBU9)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using TAP (107-10315) antibody at 1:200 dilution.
Immunohistochemical analysis of paraffin-embedded NCIN87 Xenograft , using TAP (107-10315) antibody at 1:100 dilution.
Sample (30 ug of whole cell lysate)
A: Hela
B: Molt-4
7.5% SDS PAGE
107-10315 diluted at 1:1000
A: Hela
B: Molt-4
7.5% SDS PAGE
107-10315 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.