Synonyms
C21 antibody, DC42 antibody, FLJ12894 antibody, IRA1 antibody, TBLR1 antibody, TBL1XR1 antibody, TBL1-related protein 1 antibody, transducin beta-like 1X-related protein 1 antibody, nuclear receptor co-repressor/HDAC3 complex subunit antibody, F-box-like/WD repeat-containing protein TBL1XR1 antibody, nuclear receptor corepressor/HDAC3 complex subunit TBLR1 antibody, transducin (beta)-like 1 X-linked receptor 1 antibody
Specificity
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
56 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 210 and 514 of TBLR1 (Uniprot ID# Q9BZK7)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using TBLR1 (107-10939) antibody at 1:200 dilution.

Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using TBLR1 (107-10939) antibody at 1:100 dilution.

Sample (30 ug of whole cell lysate)
A: A431
7.5% SDS PAGE
107-10939 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.