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Product Description

Specifications

Size	400 µl
Protein Name / Synonyms	TFAM (C-term)
Accession Number	Q00059
Gene Symbols	TFAM
Gene Id	7019
Concentration (lot specific)	0.5 mg/ml
Preparation	This TFAM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 216-246 amino acids from the C-terminal region of human TFAM.
Host Species	Rabbit
Clonality	Polyclonal
Species	Human

Introduction

This gene encodes a mitochondrial transcription factor that is a key activator of mitochondrial transcription as well as a participant in mitochondrial genome replication. Studies in mice have demonstrated that this gene product is required to regulate the mitochondrial genome copy number and is essential for embryonic development. A mouse model for Kearns-Sayre syndrome was produced when expression of this gene was eliminated by targeted disruption in heart and muscle cells.

Specificity

Other Names

TFAM; TCF6; TCF6L2; Transcription factor A, mitochondrial; Mitochondrial transcription factor 1; Transcription factor 6; Transcription factor 6-like 2

NCBI Accession #

NP_001257711.1;NP_003192.1

Other Accession #

NP_003192.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

29097

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100; FC: 1:10-50; IF: 1:10-50

	Western blot analysis of lysate from 293T cell line, using TFAM Antibody (C-term) (Cat# 102-10611). 102-10611 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
	TFAM antibody (C-term) (Cat# 102-10611) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TFAM antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
	TFAM Antibody (C-term) (Cat# 102-10611) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
	Fluorescent confocal image of NCI-H460 cell stained with TFAM Antibody (C-term)(Cat# 102-10611). NCI-H460 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TFAM primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).TFAM immunoreactivity is localized to mitochondrion significantly.
	TFAM Antibody (C-term) (Cat# 102-10611) western blot analysis in Hela,Jurkat,K562,MCF-7 cell line lysates (35ug/lane).This demonstrates the TFAM antibody detected the TFAM protein (arrow).