ELISA (recommended work dilution= 1:6.4x10e5(at least detecting 16 µg/ml))
Immunogen was synthetic peptide derived from Human Thyroglobulin. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum followed by Protein A/G affinity chromatography.
A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial.
Thyroglobulin (TG) represents one of the main autoantigenic targets in autoimmune thyroid disease of humans. TG is a large globular dimeric glycoprotein with a total molecular weight of 660 kDa, which occupies a key precursor role in the biosynthesis of the thyroid hormones. Approximately 75% of the total protein content of the thyroid follicle consists of TG.
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
The rabbit anti-Thyroglobulin antibody specifically detected target derived from human recombinant protein at various concentrations. Cross reactivity with mouse and rat Thyroglobulin was not tested yet
Thyroglobulin ELISA Kit, Cat# ELH-Thyroglobulin-001Chicken anti Thyroglobulin(N-terminus), CAT#130-00021
- B.Glbelli, P.Tredici, C.DE Cicco, L.Bodel, M.T. Sandri, G.Renne, R.Bruschini, N.Tradat. (2005) Preoperative determination of serum thyroglobulin to identify patients with differentiated thyroid cancer who may present recurrence without increased thyroglobulin. Acta Otorhinolaryngol Ital 25:94-99