ELISA (recommended work dilution= 1:6.4x10e5(at least detecting 16ng/ml))
Immunogen was synthetic peptide derived from Human Thyroglobulin. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum followed by Protein A/G affinity chromatography.
Supplied as lyophilized and purified antibody originally containing PBS, without Preservative Stabilizers, such as Sodium Azide. The final concentration is indicated on the shipping vial. The antibody is stable for at least years from the data of receipt when stored at -20°C to -70°C. Reconstituted antibody (recommended with H20 1 mg/ml) can also be aliquoted and stored frozen at -20°C to -70°C in a manual defrost freezer for months without detectable loss activity. Upon reconstitution, the antibody can be stored at 4°C for 1 month. Please avoid freeze-thaw cycles.
Thyroglobulin (TG) represents one of the main autoantigenic targets in autoimmune thyroid disease of humans. TG is a large globular dimeric glycoprotein with a total molecular weight of 660 kDa, which occupies a key precursor role in the biosynthesis of the thyroid hormones. Approximately 75% of the total protein content of the thyroid follicle consists of TG.
The rabbit anti-Thyroglobulin antibody specifically detected target derived from human recombinant protein at various concentrations. Cross reactivity with mouse and rat Thyroglobulin was not tested yet
Store at -20°C, if not intended for use within a month, keep it at 4°C and minimize freezing and thawing when use.
Thyroglobulin ELISA Kit, Cat# ELH-Thyroglobulin-001Chicken anti Thyroglobulin(N-terminus), CAT#130-00021
- B.Glbelli, P.Tredici, C.DE Cicco, L.Bodel, M.T. Sandri, G.Renne, R.Bruschini, N.Tradat. (2005) Preoperative determination of serum thyroglobulin to identify patients with differentiated thyroid cancer who may present recurrence without increased thyroglobulin. Acta Otorhinolaryngol Ital 25:94-99