Rabbit Anti-Human TRAP (N-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P29965 |
| Gene Id | 959 |
| Gene Symbols | CD40LG |
| Protein Name / Synonyms | TRAP (N-term) |
| Clonality | Polyclonal |
| Preparation | This TRAP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 33-62 amino acids from the N-terminal region of human TRAP. |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | Western Blotting, IF, IHC-P, FC |
| Research Area | Inflammation, Ras/Rap Signaling |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
TRAP is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in its gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome.
Specificity
Predicted Reactivity
Bovine, Pig
Other Names
CD40LG; CD40L; TNFSF5; TRAP; CD40 ligand; T-cell antigen Gp39; TNF-related activation protein; Tumor necrosis factor ligand superfamily member 5; CD_antigen=CD154; CD40 ligand, membrane form; CD40 ligand, soluble form
NCBI Accession #
NP_000065.1
Other Accession #
Q95MQ5; P51749
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
29274
Recommended Dilutions
WB: 1:1000; IHC: 1:10-50; IF: 1:10-50; FC: 1:10-50
 | Western blot analysis of TRAP antibody (N-term) (Cat# 102-16815) in NCI-H460 cell line lysates (35ug/lane). TRAP (arrow) was detected using the purified Pab. |
 | TRAP Antibody (N-term) (Cat# 102-16815) IHC analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TRAP Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated. |
 | Confocal immunofluorescent analysis of TRAP Antibody (N-term)(Cat# 102-16815) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue). |
 | TRAP antibody (N-term) (Cat# 102-16815) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.