Synonyms
AGX antibody, AGX1 antibody, AGX2 antibody, SPAG2 antibody, UAP1 antibody, sperm-associated antigen 2 antibody, sperm associated antigen 2 antibody, antigen X antibody, UDP-N-acetylhexosamine pyrophosphorylase antibody, UDP-N-acteylglucosamine pyrophosphorylase 1 antibody
Specificity
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
59 kDa
Immunogen
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 53 and 473 of UAP1
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunofluorescence analysis of paraformaldehyde-fixed A549, using UAP1(107-10391) antibody at 1:200 dilution.

Immunohistochemical analysis of paraffin-embedded ES-2 xenograft , using UAP1(107-10391) antibody at 1:500 dilution.

Sample (30 ug of whole cell lysate)
A: H1299
7.5% SDS PAGE
107-10391 diluted at 1:3000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.