UBE4B antibody [C2C3], C-Terminus, 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 10277 |
| Protein Name / Synonyms | UBE4B, C-term |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
E4 antibody, HDNB1 antibody, KIAA0684 antibody, UBOX3 antibody, UFD2 antibody, UFD2A antibody, UBE4B antibody, UFD2A-III/UBE4B-III splice isoform antibody, "ubiquitination factor E4B (UFD2 homolog, yeast) antibody", ubiquitination factor E4B (homologous to yeast UFD2) antibody, homozygously deleted in neuroblastoma 1 antibody, ubiquitin-fusion degradation protein 2 antibody, homozygously deleted in neuroblastoma-1 antibody, ubiquitin fusion degradation protein 2 antibody, ubiquitin conjugation factor E4 B antibody, homologous to yeast UFD2 antibody
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
146 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 869 and 1179 of UBE4B (Uniprot ID# O95155)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:5000-1:20000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunofluorescence analysis of methanol-fixed Hep3B, using UBE4B(107-10838) antibody at 1:500 dilution.
Immunohistochemical analysis of paraffin-embedded CA922 xenograft, using UBE4B(107-10838) antibody at 1:500 dilution.
Sample (30 ug of whole cell lysate)
A: Hep G2
5% SDS PAGE
107-10838 diluted at 1:10000
A: Hep G2
5% SDS PAGE
107-10838 diluted at 1:10000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.