UFD1L antibody, 1.02 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 7353 |
| Protein Name / Synonyms | UFD1L |
| Concentration (lot specific) | 1.02 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
UFD1 antibody, UFD1L antibody, UB fusion protein 1 antibody, ubiquitin fusion degradation protein 1 homolog antibody, ubiquitin fusion degradation 1 like (yeast) antibody
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
35 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 307 of UFD1L (Uniprot ID# Q92890)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:1000-1:10000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
UFD1L antibody detects UFD1L protein by Western blot analysis.
A. 50 µg mouse lung lysate/extract
12 % SDS-PAGE
UFD1L antibody (107-10852) dilution: 1:1000
A. 50 µg mouse lung lysate/extract
12 % SDS-PAGE
UFD1L antibody (107-10852) dilution: 1:1000
Immunofluorescence analysis of paraformaldehyde-fixed A431, using UFD1L(107-10852) antibody at 1:200 dilution.
Immunohistochemical analysis of paraffin-embedded H441 xenograft, using UFD1L(107-10852) antibody at 1:500 dilution.
Sample (30 ug of whole cell lysate)
A: A431
B: H1299
10% SDS PAGE107-10852 diluted at 1:10000
A: A431
B: H1299
10% SDS PAGE107-10852 diluted at 1:10000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.