UNC13B antibody [N3C1], Internal, 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 10497 |
| Protein Name / Synonyms | UNC13B, Internal |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
MGC133279 antibody, MGC133280 antibody, MUNC13 antibody, UNC13 antibody, Unc13h2 antibody, hmunc13 antibody, UNC13B antibody, homolog of rat Munc13 (diacylglycerol-binding) antibody, munc13-2 antibody, protein unc-13 homolog B antibody, unc-13 homolog B (C. elegans) antibody
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
181 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 680 and 957 of UNC13B (Uniprot ID# O14795)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using UNC13B(107-10955) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled at 1:2000.
Immunohistochemical analysis of paraffin-embedded A549 xenograft, using UNC13B(107-10955) antibody at 1:500 dilution.
Sample (30 ug of whole cell lysate)
A: 293T
5% SDS PAGE
107-10955 diluted at 1:500
A: 293T
5% SDS PAGE
107-10955 diluted at 1:500
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.