USP15 antibody [C3], C-Terminus, 0.87 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 9958 |
| Protein Name / Synonyms | USP15, C-term |
| Concentration (lot specific) | 0.87 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
KIAA0529 antibody, MGC131982 antibody, MGC149838 antibody, MGC74854 antibody, UNPH4 antibody, USP15 antibody, ubiquitin carboxyl-terminal hydrolase 15 antibody, deubiquitinating enzyme 15 antibody, ubiquitin thiolesterase 15 antibody, ubiquitin-specific-processing protease 15 antibody, ubiquitin thioesterase 15 antibody, ubiquitin specific peptidase 15 antibody
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
112 kDa
Immunogen
Synthetic peptide corresponding to a region within amino acids 920 and 981 of USP15 (Uniprot ID# Q9Y4E8)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunofluorescence analysis of paraformaldehyde-fixed A431, using USP15(107-10468) antibody at 1:200 dilution.
Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
7.5% SDS PAGE
107-10468 diluted at 1:1000
A: NIH-3T3
B: JC
C: BCL-1
7.5% SDS PAGE
107-10468 diluted at 1:1000
Immunohistochemical analysis of paraffin-embedded N87 xenograft, using USP15(107-10468) antibody at 1:100 dilution.
Sample (30 ug of whole cell lysate)
A: Molt-4
7.5% SDS PAGE
107-10468 diluted at 1:1000
A: Molt-4
7.5% SDS PAGE
107-10468 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.