Synonyms
DKFZp686C13257 antibody, FLJ20727 antibody, TRFP antibody, USP47 antibody, ubiquitin carboxyl-terminal hydrolase 47 antibody, ubiquitin-specific-processing protease 47 antibody, Trf (TATA binding protein-related factor)-proximal homolog antibody, ubiquitin specific protease 47 antibody, deubiquitinating enzyme 47 antibody, ubiquitin thiolesterase 47 antibody, ubiquitin thioesterase 47 antibody, ubiquitin specific peptidase 47 antibody
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
157 kDa
Immunogen
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 635 and 870 of USP47
Applications
Applications Tested
ICC/IF,Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunofluorescence analysis of methanol-fixed HeLa, using USP47(107-10595) antibody at 1:200 dilution.

Sample (50 ug of whole cell lysate)
A: Mouse brain
5% SDS PAGE
107-10595 diluted at 1:1000

Sample (30 ug of whole cell lysate)
A: Hela
5% SDS PAGE
USP47 antibody
107-10595 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.