Synonyms
MGC3745 antibody, VAP-33 antibody, VAP-A antibody, VAP33 antibody, hVAP-33 antibody, VAPA antibody, VAMP-A antibody, 33 kDa VAMP-associated protein antibody, vesicle-associated membrane protein-associated protein A antibody, VAMP-associated protein A antibody, "VAMP (vesicle-associated membrane protein)-associated protein A, 33kDa antibody"
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
28 kDa
Immunogen
Synthetic peptide corresponding to a region within amino acids 29 and 122 of VAPA (Uniprot ID# Q9P0L0)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunohistochemical analysis of paraffin-embedded lung AdCA, using VAPA(107-10986) antibody at 1:500 dilution.

Immunohistochemical analysis of paraffin-embedded breast ca, using VAPA(107-10986) antibody at 1:500 dilution.

Sample (30 ug of whole cell lysate)
A: H1299
12% SDS PAGE
107-10986 diluted at 1:1000

Sample (30 ug of whole cell lysate)
A: NIH-3T3
12% SDS PAGE
VAPA antibody
107-10986 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.