VDAC1 antibody [N1C2], 0.35 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 7416 |
| Protein Name / Synonyms | VDAC1 |
| Concentration (lot specific) | 0.35 mg/ml |
| Recommended Applications | IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
MGC111064 antibody, PORIN antibody, VDAC-1 antibody, VDAC1 antibody, outer mitochondrial membrane protein porin 1 antibody, voltage-dependent anion-selective channel protein 1 antibody, porin 31HM antibody, plasmalemmal porin antibody, porin 31HL antibody, voltage-dependent anion channel 1 antibody
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
31 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 211 of VDAC1 (Uniprot ID# P21796)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using VDAC1(107-11115) antibody at 1:500 dilution.
VDAC1 antibody [N1C2] detects VDAC1 protein at mitochondria on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
VDAC1 antibody [N1C2] (107-11115) dilution: 1:500.
Sample: Paraffin-embedded mouse muscle.
VDAC1 antibody [N1C2] (107-11115) dilution: 1:500.
Sample (30 ug of whole cell lysate)
A: A431
12% SDS PAGE
VDAC1 antibody
107-11115 diluted at 1:1000
A: A431
12% SDS PAGE
VDAC1 antibody
107-11115 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.